By Andrei G. Pakhomov, Damijan Miklavcic, Marko S. Markov
"A mirrored image of the serious learn of the results of electromagnetic fields on dwelling tissues that has taken position over the last numerous a long time, this publication discusses the theoretical and experimental facts and issues the results of sturdy electromagnetic fields and/or electrical pulses and their value in drugs and biology. The authors current the elemental options utilized in electroporation and the complicated tools for construction of nanopores, highlighting their uncomplicated technological know-how and scientific functions. subject matters contain nano electroporation, vintage electroporaiton, experimental facts for electroporation of dwelling cells, and electroporation for melanoma and wound healing"--Provided by means of writer. learn more... content material: fundamentals of Electroporation actual Chemical concept of Membrane Electroporation and Electrotransfer of Biogenic brokers, E. Neumann and S. Kakorin Bioelectric influence of extreme Nanosecond Pulses, K.H. Schoenbach precipitated Transmembrane Voltage-Theory, Modeling, and Experiments, T. Kotnik and G. Pucihar Electroporation: A evaluate of easy difficulties in thought and scan, M.S. Markov Mechanisms of Electroporation in Lipid platforms Electrodeformation, Electroporation, and Electrofusion of Cell-Sized Lipid Vesicles, R. Dimova Fluorescent tools in overview of Nanopore Conductivity and Their Computational Validation, M. Kotulska, W. Dyrka, and P. Sadowski Electroporation of Lipid Membranes: Insights from Molecular Dynamics Simulations, M. Tarek and L. Delemotte Nanoscale Restructuring of Lipid Bilayers in Nanosecond electrical Fields, P.T. Vernier Mechanisms of Electroporation of Cells Nanopores: a unique Transmembrane Passageway in Electroporated Cells, A.G. Pakhomov and O.N. Pakhomova version of mobile Membrane Electroporation and Transmembrane Molecular delivery, D. Miklavcic and L. Towhidi Kinetics of Pore Formation and Disappearance within the cellphone in the course of Electroporation, G. Saulis the heart beat Intensity-Duration Dependency for cellphone Membrane Electroporation, D. Miklavcic, G. Pucihar, A.M. Lebar, J. Krmelj, and L. Towhidi Mechanisms of Electroporation in Tissues Drug-Free, reliable Tumor Ablation by way of Electroporating Pulses: Mechanisms That Couple to Necrotic and Apoptotic mobilephone loss of life Pathways, A.T. Esser, K.C. Smith, T.R. Gowrishankar, and J.C. Weaver Gene Electrotransfer: From uncomplicated procedures to Preclinical purposes, J.-M. Escoffre, A. Paganin-Gioanni, E. Bellard, M. Golzio, M.-P. Rols, and J. Teissie Technical concerns Modeling electrical box Distribution In Vivo, N. Pavselj, A. Zupanic, and D. Miklavcic innovations of Electroporation Pulse new release and review of electrical Pulse turbines for phone and Tissue Electroporation, M. Rebersek and D. Miklavcic iteration of Ultrashort Pulses, J.F. Kolb Nanosecond Pulsed electrical box supply to organic Samples: problems and strength suggestions, A. Silve, J. Villemejane, V. Joubert, A. Ivorra, and L.M. Mir functions of Electroporation Translation of Electroporation-Mediated DNA supply to the hospital, L.C. Heller and R. Heller medical Electrochemotherapy: The Italian adventure, C.R. Rossi and L.G. Campana Tumor Blood Flow-Modifying results of Electroporation and Electrochemotherapy-Experimental facts and Implications for the treatment, T. Jarm, M. Cemazar, and G. Sersa lectrochemotherapy as a part of an Immunotherapy process within the therapy of melanoma, J. Gehl ombined electric box and Ultrasound: A Nondrug-Based strategy for Tumor Ablation, P.F. Forde, C. Twomey, G.C. O' Sullivan, and D. M. Soden mixed Modality remedy: Electrochemotherapy with Tumor Irradiation, G. Sersa, S. Kranjc, and M. Cemazar Irreversible Electroporation in drugs, B. Rubinsky foodstuff and Biomaterials Processing Assisted via Electroporation, N. Lebovka and E. Vorobiev In Vivo Electroporation: an enormous damage Mechanism in electric surprise Trauma, I. Barakat, J. Gallaher, H. Chen, and R.C. Lee Index summary: Reflecting the serious research of the results of electromagnetic fields on dwelling tissues that has taken position through the years, this name summarizes the experimental findings and theories regarding permeabilization of biomembranes through pulsed electrical fields. it truly is meant generally for biomedical and actual scientists, engineers, and clinicians. learn more...
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Additional resources for Advanced electroporation techniques in biology and medicine
The experiments with 60 ns pulses showed, for the first time, that poration/disruption of intracellular membranes can be achieved with ultrashort electrical pulses without the loss of the surface membrane integrity. Experiments with shorter pulses (10 ns) have confirmed the earlier results (Buescher and Schoenbach, 2003). Similar experimental results have been reported by Tekle et al. (2005). A mixed population of phospholipid vesicles and single COS-7 cells, in which vacuoles were induced by stimulated endocytosis, were exposed to nanosecond pulses.
The degree of fragmentation was found to be linearly proportional to the square root of the pulse number. At this time, it is not known if DNA damage is a primary (direct) effect of intense nanosecond electric fields or a secondary effect caused by membrane permeabilization. , 2006). , 2004). 15. This process of aggregation is initiated by calcium release from internal stores. This is consistent with the well-known fact that aggregation of platelets by known agonists, such as thrombin, requires an increase in intracellular free calcium.
This observation was exciting in that the application of nsPEFs can be considered to be a way of treating tumors without the additional requirement of chemotherapeutic agents, with no residual side effects following treatment and with limited inflammation at the treatment site. © 2010 by Taylor and Francis Group, LLC 39 Bioelectric Effect of Intense Nanosecond Pulses Nuccitelli et al. (2006) confirmed this hypothesis. It was found that electric pulses (300 ns in duration and 40 kV/cm) can cause a total remission of skin cancer in mice.
Advanced electroporation techniques in biology and medicine by Andrei G. Pakhomov, Damijan Miklavcic, Marko S. Markov