Antibody Expression and Engineering by Henry Y. Wang and Tadayuki Imanaka (Eds.) PDF

By Henry Y. Wang and Tadayuki Imanaka (Eds.)

ISBN-10: 0841215294

ISBN-13: 9780841215290

ISBN-10: 0841233144

ISBN-13: 9780841233140

content material: construction of engineered antibodies in myeloma and hybridoma cells : improvements in gene expression and approach layout / D.K. Robinson, D. DiStefano, S.L. Gould, G. Cuca, T.C. Seamans, D. Benincasa, S. Munshi, C.P. Chan, J. Stafford-Hollis, G.F. Hollis, D. Jain, ok. Ramasubramanyan, G.E. Mark, and M. Siberklang --
Antibody construction in human hybridomas with growth-associated construction kinetics / Hidekazu Sawada and Kazuaki Kitano --
Antibody creation in chinese language hamster ovary cells utilizing an impaired selectable marker / R.S. Barnett, K.L. Limoli, T.B. Huynh, E.A. Ople, and M.E. Reff --
Antibody construction in insect cells / Kathleen N. Potter, Yucheng Li, and J. Donald Capra --
Antibody expression in crops / J. K-C. Ma --
Engineering of immunoglobulin Fc and single-chain Fv proteins in Escherichia coli / David Filpula, Michele Rollence, Nina Essig, James Nagle, Aniruddha Achari, and Timothy Lee --
T-cell specific immunofusion proteins from Escherichia coli : motive, layout, and houses / Marc higher and Patricia A. Nolan --
construction and purification of antibody utilizing Bacillus subtilis as an expression host / S.L. Wong, X.C. Wu, L.P. Yang, S.C. Ng, and N. Hudson --
Trichoderma reesei, a promising novel host for antibody construction / Eini Nyyssönen and Sirkka Keränen --
utilising antibody catalysis to natural synthesis / Brian J. Lavey and Kim D. Janda --
robust and particular peroxidase job with an antibody L chain-porphyrin Fe(III) complicated / Tadayuki Imanaka and Masahiro Takagi.

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1%) expressed less than 25 ng/ml of antibody. 0 χ 10 cells/electroporation) of the TCAE vector, which contains a consensus Kozak sequence at the translation initiation site, yielded 258 G418 resistant colonies. There were no colonies in this experiment that produced greater than 1000 ng/ml of antibody. Two hundred and one (201) of these colonies (78%) expressed less than 25 ng/ml of antibody. In other experiments where equal amounts of DNA of the two vectors (TCAE, ANEX) were transfected simultaneously, the ANEX vector consistendy yielded a 80-90% reduction in numbers of G418 resistant clones per electroporation.

As a result of comparison of various culture methods, we decided that short-term perfusion culture should be a practical method for h-h hybridomas with growth-associated production kinetics. 29AGC-1 was performed under short-term perfusion using a 50-L fermentor, and the purification of IgG-MoAb was efficiendy carried out in yields of approximately 75% on the scale of more than 100L of culture supernatant. These IgG MoAbs neutralized HBV infectivity and protected chimpanzees against the development of hepatitis B.

BARNETT ET AL. ch003 1 2 3 4 Figure 8 Shown is a Northern blot of RNA isolated from a G418 resistant TCAE clone and NEOSPLA clone. Both clones secreted high amounts of chimeric antibody. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1995. ch003 1 2 3 4 5 Figure 9 Shown is a Southern Blot of DNA isolated from a CHO clone integrated into a 'hot spot' and the derived 5nM and 50nM MTX clones. 5 mg of high molecular weight DNA was digested with Nhe I which cuts once in each plasmid and gives two bands of unknown size for each integration site into cellular DNA.

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Antibody Expression and Engineering by Henry Y. Wang and Tadayuki Imanaka (Eds.)


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